Reaggregate Thymus Cultures

Abstract: In the thymus, immature CD4+8+ thymocytes expressing randomly rearranged Tcell receptor α and βchain genes undergo positive and negative selection events based on their ability to recognize selfpeptide, major histocompatibility complex (MHC) molecules expressed by thymic stromal cells. In vivo analysis of the role of thymic stromal cells during intrathymic selection is made difficult by the cellular complexity of the thymic microenvironment in the steadystate adult thymus,