Conférence E. CHARPENTIER Le CRISPR Cas9 La révolution de lingénierie génomique

Conférence de Emmanuelle CHARPENTIER intitulée The CRISPRCas9 revolution in genome engineering: lessons learned from bacteria 00:00:00 Introduction 00:01:10 Understand diseases at the fundamental level 00:04:16 Jacques Monod (19101976) 00:05:30 Important milestones towards gene therapy 00:10:09 The CRISPR craze 00:10:56 Bacterial pathogens 00:11:26 Understand bacteria, host interactions 00:11:52 Infection of bacteria by invading genomes 00:14:14 The CRISPRCas adaptive immune system 00:17:37 Spacer acquisition 00:19:04 crRNA expression and maturation 00:20:06 interference 00:23:01 mecanisms 00:23:06 CRISPRCas has evolved into five major types 00:24:02 Sans titre 00:26:19 CRISPRCas9 technology: a twocomponent system 00:29:04 CRISPRCas9 pathway in bacteria 00:29:09 Type II CRISPRCas locus 00:29:30 Type II CRISPRCas: model of crRNA maturation 00:30:22 Type II CRISPRCas: model of DNA targeting 00:30:38 Type II CRISPRCas