4. Genetic Engineering (cont. )

Frontiers of Biomedical Engineering (BENG 100) Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: antisense therapy and RNA interference. Molecular cloning techniques to massproduce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in